Toxicity of cationic liposome Lipofectamine 2000 in human pancreatic cancer Capan-2 cells.
- Author:
Ying-Qiang ZHONG
1
;
Jing WEI
;
Yu-Ru FU
;
Jing SHAO
;
Yi-Wen LIANG
;
Yan-Hua LIN
;
Juan LIU
;
Zhao-Hua ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cations; toxicity; Cell Cycle; drug effects; Cell Line, Tumor; Humans; Lipids; genetics; toxicity; Liposomes; toxicity; Pancreatic Neoplasms; pathology; RNA, Small Interfering; genetics; Transfection
- From: Journal of Southern Medical University 2008;28(11):1981-1984
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the toxicity of cationic liposome Lipofectamine 2000 (Lipo) in human pancreatic cancer Capan-2 cells.
METHODSCapan-2 cells were cultured in the presence of Lipo at toxic concentrations, and the cell growth, apoptosis and cell cycle changes were evaluated by cell counting and flow cytometry.
RESULTSThe concentrations of both Lipo and siRNA affected the transfection efficiency. In a transfection volume of 2 ml, the presence of 5 microl Lipo resulted in slowed growth of Capan-2 cells, which was especially obvious after 3 days (P<0.001). Prolonged culture of the transfected cells caused significant increases in early apoptotic cells (P<0.05) and in the damaged or necrotic cells (P<0.001), and resulted in reduced viable cells (P<0.01); these changes became obvious after a 48-hour culture, which also increased the ratio of G(0)/G(1) phase cells (P<0.05) and decreased those of G(2)/M phase cells (P<0.01), S phase cells (P<0.01), and the late apoptotic cells (P<0.05).
CONCLUSIONToxic concentrations of Lipo can affect the growth, apoptosis and cell cycles of Capan-2 cells in vitro, and this urges careful concentration selection when using Lipo for gene transfer into different cells.