Determination of plasma escitalopram with liquid chromatography-mass spectrometry.
- Author:
Heng ZHENG
1
;
Ling YANG
;
Wen-Yan TANG
Author Information
- Publication Type:Journal Article
- MeSH: Antidepressive Agents, Second-Generation; blood; pharmacokinetics; Chromatography, High Pressure Liquid; methods; Citalopram; blood; pharmacokinetics; Humans; Sensitivity and Specificity; Tandem Mass Spectrometry; methods
- From: Journal of Southern Medical University 2008;28(11):2044-2046
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an liquid chromatography-mass spectrometry (HPLC-MS/MS)-based method for determining escitalopram in human plasma.
METHODSA liquid-liquid ether extraction approach was adopted using dextromethorphan as the internal standard. The Agilent ZORBAX SB-C18 (3.5 microm,2.1 x 150 mm) was used as the analytical column with acetonitrile-NH4AC buffer (70:30, V/V) as the mobile phase at the flow rate of 0.3 ml/min. The sample was ionized by electrospray ionization source in the triple quadruple tandem mass spectrometer, and the plasma escitalopram determined with a multiple reaction monitoring mode of m/z325.4-->109.4.
RESULTSThe linear range was 0.0866-64.13 microg/L (r=0.9965) for escitalopram in human plasma, with the absolute recovery between 64.98% and 78.72% and the within-day and between-day deviations less than 10%.
CONCLUSIONThe method is sensitive, accurate, rapid, specific and well applicable for clinical pharmacokinetics study of escitalopram.
