Effects of resveratrol on the release of Glu and Gln in cultured rat astrocytes with stretch injury.
- Author:
Hao ZHOU
1
;
Qing CHEN
;
Jiang GUO
;
Qian WANG
;
Dan-li KONG
;
Shou-yi YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Astrocytes; cytology; metabolism; Cells, Cultured; Glutamine; metabolism; Glutathione; metabolism; L-Lactate Dehydrogenase; metabolism; Neuroprotective Agents; pharmacology; Rats; Rats, Sprague-Dawley; Stilbenes; pharmacology; Stress, Mechanical
- From: Journal of Southern Medical University 2008;28(12):2215-2217
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of resveratrol on amino acid release in cultured astrocytes with stretch injury and explore its possible mechanism for central nervous system protection.
METHODSCultured rat astrocytes were treated with different concentrations of resveratrol for 12 h and the subsequent changes in Glu and Gln release and lactate dehydrogenase(LDH) leakage were examined after stretch injury.
RESULTSHigh-performance liquid chromatography showed that stretch injury increased Glu release from the astrocytes (P<0.05), and at the concentration of 1 micromol/L, resveratrol further increased Glu release (P<0.05), whereas at 100 micromol/L, resveratrol obviously inhibit Glu release (P<0.05). LDH leakage increased significantly after stretch injury of the astrocytes (P<0.05), and resveratrol acted to further increase LDH leakage at 1 micromol/L but inhibited LDH leakage at 100 micromol/L (P<0.05).
CONCLUSIONResveratrol can inhibit the release of Glu and LDH leakage from rat astrocytes to protect the cells from stretch injury.
