Genotyping and its epidemiological significance on Yunnan Yersinia pestis under Fse I enzyme digestion method.

Liyuan Shi; Rui YE; Shanshan Dong; Ying Guo; Guangcan Yang; Rong Zhang; Zhigang Cui; Wei LI; Peng Wang

Return

Genotyping and its epidemiological significance on Yunnan Yersinia pestis under Fse I enzyme digestion method.

Author: Liyuan SHI ¹ ; Rui YE ² ; Shanshan DONG ¹ ; Ying GUO ¹ ; Guangcan YANG ¹ ; Rong ZHANG ³ ; Zhigang CUI ⁴ ; Wei LI ⁴ ; Peng WANG ⁵ ;
Author Information

1. Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, China.
2. School of Public Health, Dali University, Dali, Yunnan Province.
3. School of Public Health,Dali University, Dali, Yunnan Province.
4. National Institute for Communicable Disease Control and Prevention and State Key Laboratory for Infectious Diseases Prevention and Control, Chinese Center for Disease Control and Prevention.
5. Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, China
Publication Type:Journal Article
MeSH: Animals; China; epidemiology; Cluster Analysis; Electrophoresis, Gel, Pulsed-Field; Genotype; Humans; Phylogeny; Plague; epidemiology; Rats; Yersinia pestis; genetics; isolation & purification
From: Chinese Journal of Epidemiology 2014;35(2):182-185
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo create the fingerprint library of Yunnan Yersinia pestis by pulse field gel electrophoresis(PFGE)with Fse I enzyme digestion method and to study its epidemiological significance.
METHODSWe used rare cutting restriction enzyme Fse? to digest Yunnan Yersinia pestis strains that were isolated from foci including Rattus flavipectus Plague Focus,Apodemus chevrieri-Eothenomys miletus Plague Focus and Yulong Plague Focus. Fingerprints to Bionumerics software were used for cluster analysis.
RESULTS30 tested strains were divided into 16 genotypes with the similarity value as 79.8%-100.0% . 16 genotypes wee formed into 4 clusters, with one consisted of only EV76 while the other three belonged to Rattus flavipectus,Apodemus chevrieri- Eothenomys miletus and Yulong clusters, respectively.
CONCLUSIONSPFGE genotypes of Yunnan Yersinia pestis accorded with its ecotypes and biovars, with clustered regional features. The strains isolated from Yulong showed an unique PFGE type, indicating a new clone group was identified.