Granulocyte-macrophage colony-stimulating factor and interleukin 4 induce the malignant transformation of the bone marrow-derived human adult mesenchymal stem cells.
- Author:
Xiao-Gang ZHOU
1
;
Yi YANG
;
Jin-Song YANG
;
Jian ZHOU
;
Tao-Lin FANG
;
Wen-da DAI
;
Zheng-Rong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Bone Marrow Cells; cytology; drug effects; Cell Transformation, Neoplastic; drug effects; Cells, Cultured; Flow Cytometry; Granulocyte-Macrophage Colony-Stimulating Factor; pharmacology; Humans; Immunohistochemistry; Interleukin-4; pharmacology; Mesenchymal Stromal Cells; cytology; drug effects
- From: Chinese Medical Journal 2011;124(5):729-733
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDThe purpose of the study was to examine the effects of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4) on the bone-marrow-derived human adult mesenchymal stem cells (hMSCs).
METHODSThe hMSCs were isolated and cultured with GM-CSF and IL-4 for a period of one month. A single colony of transformed cells was then isolated and their phenotype was characterized by morphology, surface marker expression, and in vivo tumorigenesis.
RESULTSAfter one month culture, the transformed mesenchymal cells exhibited the morphology and phenotype similar to those of tumor cells, and also caused multiple fast growing lung deposits when it was injected into immunodeficient mice.
CONCLUSIONCytokines-driven malignant transformation of hMSCs may be a useful model for studying signaling pathways initiating malignant transformation of hMSC.
