Regulating function of coenzymization and decoenzymization of the lactate dehydrogenase isozymes in the mouse tissues during hypoxia.
- Author:
Yuan MINGXIU
1
;
Jiang HUI
;
Deng AIPING
;
Zhou QINGZHONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Hindlimb; enzymology; Hypoxia; enzymology; Isoenzymes; metabolism; L-Lactate Dehydrogenase; classification; metabolism; Liver; enzymology; Mice; Muscles; enzymology; Myocardium; enzymology; Random Allocation
- From: Chinese Medical Sciences Journal 2003;18(2):128-131
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the characteristics of changes of LDH enzyme patterns of mice under slight hypoxia.
METHODSMice treated with artificial hypoxia, various tissues were made for the test of LDH enzymatic activity by the specific staining technique. LDH (1-5) relative percentage enzymatic activity (RPEA) were measured with CS-910 dual-wavelength thin layer chromatography scanner.
RESULTSThe RPEA of LDH isozymes of various tissues after slight hypoxia shifted to the isozymes LDH1 and LDH2, whose principal subunits are H subunits, and the RPEA of LDH1 (H4), LDH2 (H3M) increased, while RPEA of LDH5 (M4) in various tissues decreased prominently except the cardiac muscle, and that of LDH4 (HM3) decreased as well. After polyacrylamide gel electrophoresis (PAGE) of the hypoxia treated cardiac muscle specimen was made, activity subbands originated regularly in the isoyme patterns of LDH, with the regularity of LDH1 (0 subband), LDH2 (0-1 subbands), LDH3 (0-2 subbands), LDH4 (1-3 subbands), LDH5 (2-4 subbands). After adding appropriate amount of NAD+ to the hypoxia treated cardiac muscle specimen, PAGE showed the subbands of four isoymes (LDH2-LDH5) reduced or even totally disappeared in the isozyme patterns.
CONCLUSIONSThe negative feedback regulation of coenzymization and decoenzymization of LDH isozymes is one of the mouse stress responses to slight hypoxia.
