Protective effect of Shenxiong injection on cerebral ischemia-reperfusion injury of rats.
- Author:
Xing-Yu WANG
;
Yu-Yan ZHANG
;
Hai-Tong WAN
;
Tao ZHAO
;
Wei FU
;
Hui-Fen ZHOU
;
Peng ZHOU
;
Ze-Feng WANG
;
Jie-Hong YANG
- Publication Type:Journal Article
- MeSH: Animals; Brain; blood supply; drug effects; metabolism; Brain Ischemia; complications; Drugs, Chinese Herbal; administration & dosage; pharmacology; therapeutic use; Injections; L-Lactate Dehydrogenase; metabolism; Male; Malondialdehyde; metabolism; Nitric Oxide; metabolism; Nitric Oxide Synthase; metabolism; Rats; Rats, Sprague-Dawley; Reperfusion Injury; complications; drug therapy; enzymology; metabolism; Superoxide Dismutase; metabolism
- From: China Journal of Chinese Materia Medica 2014;39(3):503-506
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the protective effect of Shenxiong injection on the cerebral ischemia-reperfusion injury of senile rats.
METHODTotally 108 Sprague-Dawley (SD) rats were randomly divided into the sham operation group, the model group, the Ni-modipine group and Shenxiong injection groups (low, middle, and high doses). The rat brain ischemia-reperfusion model was established by the middle cerebral artery occlusion (MCAO) method in rats, in order to observe the effect of Shenxiong injection on neurological score and brain infarct volume of rats with cerebral ischemia-reperfusion injury, and determine the contents of NOS, NO, SOD, MDA and LDH in brain tissues. The contents of TNF-alpha and IL-1beta levels in brain tissues were measured by enzyme-linked immunosorbent assay (ELISA) method.
RESULTShenxiong injection could significantly decrease neurological score, injury degree of brain tissues and brain infarct volume of rats with cerebral ischemia-reperfusion injury, increase the vigor of SOD, decrease the levels of MDA, NO, NOS and LDH, and inhibit IL-1beta and TNF-alpha expressions.
CONCLUSIONShenxiong injection has the obvious protective effect on the brain ischemia-reperfusion injury in rats. Its mechanism may be related to the improvement of neurological function, the reduction of free radical injury, and the inhibition of inflammation factor expression.