Preparation of novel gypenosides long-circulating liposomes consisted by sphingomyelin and beta-sitosterol modified by PEG.
- Author:
Fan YU
;
Jing-Ming YANG
;
Jin-Juan LI
- Publication Type:Journal Article
- MeSH: Drug Compounding; methods; Feasibility Studies; Gynostemma; chemistry; Liposomes; blood; chemistry; Plant Extracts; chemistry; Polyethylene Glycols; chemistry; Reproducibility of Results; Sitosterols; chemistry; Sphingomyelins; chemistry
- From: China Journal of Chinese Materia Medica 2014;39(6):997-1001
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the feasibility of preparing novel gypenosides long-circulating liposomes with PEG grafted on beta-sitosterol (PEG-Sito).
METHODSuccinicanhydride was adopted to connect beta-sitosterol and PEG 2000. Sphingomyelin and PEG-Sito was used as material to prepare gypenosides long-circulating liposomes by using ethanol injection method. Encapsulation efficiency was determined by using protamine precipitation method. H-NMR was used to verify the synthesis of PEG-Sito, the novel gypenosides long-circulating liposomes were characterized by particle size, zeta potential and atomic force microscope.
RESULTThe synthesis of PEG-Sito was verified by 1H-NMR. Encapsulation efficiency of long-circulating liposomes prepared by ethanol injection method was 74.3%, particle size was 288.1 nm, zeta potential was -20.25 mV, the morphology were round observed by AFM.
CONCLUSIONThe novel gypenosides long-circulating liposomes prepared with PEG-Sito was feasible, it had a high encapsulation efficiency and good morphology.