Study on preparation process and formulation optimization of herpetin liposomes.
- Author:
Xin ZHANG
;
Rui TAN
;
Jian GU
;
Li-Li HE
;
Li-Na FAN
;
Xing-Mei NAN
- Publication Type:Journal Article
- MeSH:
Chemistry, Pharmaceutical;
methods;
Cholesterol;
chemistry;
Drug Compounding;
methods;
Drugs, Chinese Herbal;
chemistry;
Lignans;
chemistry;
isolation & purification;
Liposomes;
chemistry;
Phospholipids;
chemistry;
Ultrasonics
- From:
China Journal of Chinese Materia Medica
2014;39(6):1007-1010
- CountryChina
- Language:Chinese
-
Abstract:
Herpetin (HPT) is an active monomer constituent isolated from lignanoid in seeds of Herpetospermum caudigerum. HPT shows inhibitory effects in hepatic injury and HBV-DNA and the replication. In the study, we successfully prepare herpetin liposomes by film dispersion method for the first time. The prescription process was optimized, with the entrapment efficiency as the index. According to the optimized prescription, the mass ratio of HPT: phospholipids: cholesterol was 2.44:78.05: 19.51, the hydration and de-molding process was performed with 0.5% F68 solution at 50 degrees C, and the water-bath ultrasonic time was 20 min. The HPT liposomes prepared by this method showed an average entrapment efficiency of (94.50 +/- 2.15)% and a particle size of (119.2 +/- 10.7) nm, which was consistent with the trial expectations and will lay a solid foundation for the hepatic targeting delivery system in future.
