Determination of five components in rongxin pills by HPLC.
- Author:
Zhong-Ying CHU
;
Li-Hong WU
;
Lan TIAN
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
Cyclooctanes;
analysis;
Diterpenes, Abietane;
analysis;
Drugs, Chinese Herbal;
chemistry;
Lignans;
analysis;
Linear Models;
Organic Chemicals;
analysis;
Phenanthrenes;
analysis;
Polycyclic Compounds;
analysis;
Quality Control;
Reproducibility of Results
- From:
China Journal of Chinese Materia Medica
2014;39(6):1016-1019
- CountryChina
- Language:Chinese
-
Abstract:
To establish a method for determination contents of schizandrin, tanshinone I, cryptotanshinone, tanshinone II (A) and schizandrin B in rongxin pills. The HPLC method was performed on an Agilent C18. The mobile phase was composed of methnol and water wish gradient elution. The flow rate was 1.0 mL x min(-1). The column temperature was 30 degrees C and the detection wavelength wash 240 nm. The linear of schizandrin, tanshinone I, cryptotanshinone, Tanshinone II (A) and schizandrin B were 3.000-48.00 (r = 1.000), 3.985-63.76 (r = 0.999 9), 6.370-101.9 (r = 1.000), 8.690-139.0 (r = 0.999 9), 1.700-27.20 mg x L(-1) (r = 0.999 9), respectively. The average recoveries were 98.44%, 100.3%, 99.29%, 99.07%, 98.42%, and RSDs were 0.61%, 1.1%, 0.52%, 0.72%, 0.97%. The method is convenient, accurate and has good precision. It can be used for determination of the preparation.
