Protective effect of jiedu tongluo injection on cerebral edema in rats with lesion of cerebral ischemia/reperfusion.
- Author:
Li-Fei WU
;
Yue XING
;
Ya-Lan GUAN
;
Zhen-Quan LIU
;
Wen-Sheng ZHANG
- Publication Type:Journal Article
- MeSH: Animals; Blood-Brain Barrier; drug effects; metabolism; Brain Edema; etiology; metabolism; prevention & control; Brain Ischemia; complications; Drugs, Chinese Herbal; administration & dosage; pharmacology; E-Selectin; metabolism; Evans Blue; metabolism; Gene Expression Regulation, Enzymologic; drug effects; Injections; Intercellular Adhesion Molecule-1; metabolism; Male; Matrix Metalloproteinase 9; metabolism; Permeability; drug effects; Rats; Rats, Sprague-Dawley; Reperfusion Injury; complications; Vascular Cell Adhesion Molecule-1; metabolism
- From: China Journal of Chinese Materia Medica 2014;39(6):1088-1092
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effects of Jiedu Tongluo injection on cerebral edema induced by focal lesion of cerebral ischemia/reperfusion, the hydrous content of brain and the expressions of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), E-selectin and MMP-9 in rats.
METHODThe model of brain middle cerebral artery ischemia/reperfusion was established by the thread approach. After 24 hours of reperfusion, cerebral edema formation was determined by the hydrous content of brain. The permeability of blood brain barrier was evaluated based on the leakage of Evans blue. Enzyme-linked immunoadsordent assay (ELISA)was used to examine the expression of ICAM-1, VCAM-1, E-selectin. The expression of MMP-9 was measured by immunohistochemistry.
RESULTJDTL, in the dose of 2 mL x kg(-1) and 4 mL x kg(-1), relieved cerebral edema (P < 0.05, P < 0.01), reduced the expressions of ICAM-1, VCAM-land E-selectin and decreased MMP-9 activity (P < 0. 05, P < 0.01) in model rats.
CONCLUSIONJiedu Tongluo injection has a protective effect on rat brain from cerebral edema induced by the injury of focal cerebral ischemia/reperfusion. The mechanism is related to that Jiedu Tongluo injection can reduce the expressions of ICAM-1, VCAM-1 and E-selectin and inhibit of MMP-9 activation in rat brain.