Effect of sodium tanshinone II (A) sulfonate on Ang II -induced atrial fibroblast collagen synthesis and TGF-beta1 activation.
- Author:
Le YANG
;
Xiao-Jing ZOU
;
Zhao YIN
;
Hong-Zhen HAO
- Publication Type:Journal Article
- MeSH: Angiotensin II; pharmacology; Animals; Collagen; biosynthesis; Fibroblasts; cytology; drug effects; metabolism; Gene Expression Regulation; drug effects; Heart Atria; cytology; Phenanthrenes; pharmacology; Rats; Rats, Wistar; Signal Transduction; drug effects; Thrombospondin 1; metabolism; Transforming Growth Factor beta1; metabolism
- From: China Journal of Chinese Materia Medica 2014;39(6):1093-1096
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of sodium tanshinone II (A) sulfonate (STS) on Ang II -induced atrial fibroblast collagen synthesis and TGF-beta1 activation.
METHODAtrial fibroblasts of neonatal rats were cultured to determine the content of collagen protein. The original synthesis rate determined by the [3H]-proline incorporation method was taken as the index for myocardial fibrosis. The content of active TGF-beta1 and total TGF-beta1 in cell culture supernatants were tested and cultured by ELISA. The expression of thrombospondin-1 (TSP-1) was assessed by using Western blot.
RESULTAng II could significantly increase the content of atrial fibroblast collagen and the collagen synthesis rate, the TSP-1 expression and the concentration of active TGF-beta1, without any obvious change in total TGF-beta1. After the STS treatment, all of the indexes, apart from total TGF-beta1, were obviously down-regulated.
CONCLUSIONSTS could decrease the secretion of Ang II -induced atrial fibroblast collagen and the synthesis rate. Its mechanism is related to the inhibition of TSP-1/TGF-beta1 pathway.