HPLC-fingerprint-based quality evaluation on a Tibetan medicine Phyllanthus emblica and its tannin parts.
- Author:
Xue-Fei SUN
;
Hong-Yan ZHANG
;
Qing XIA
;
Hai-Juan ZHAO
;
Ling-Fang WU
;
Lan-Zhen ZHANG
;
Ren-Bing SHI
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
analysis;
Medicine, Tibetan Traditional;
Phyllanthus emblica;
chemistry;
classification;
Quality Control;
Tannins;
analysis;
Tibet
- From:
China Journal of Chinese Materia Medica
2014;39(7):1173-1178
- CountryChina
- Language:Chinese
-
Abstract:
This study is to establish the fingerprint for Phyllanthus emblica and their tannin parts from different habitats by HPLC for its quality control. The determination was carried out on a Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column, with methanol-0.2% glacial acetic acid as mobile phase with gradient elution at a flow rate of 1 mL x min(-1). The temperature was maintained at 30 degrees C and the detected wavelength is 260 nm, Thirteen chromatographic peaks were extracted as the common peaks of the fingerprint of P. emblica, and eleven as the common peaks of P. emblica tannin parts, and five peaks were identified by comparing with referent samples. The fingerprints of 8 samples were compared and classified by similarity evaluation, cluster analysis and principal component analysis (PCA). The similarity degrees of eight P. emblica were between 0.763 and 0.993, while tannin parts were between 0.903 and 0.991. All the samples of P. emblica and their tannin parts were classified into 3 categories. The method was so highly reproducible, simple and reliable that it could provide basis for quality control and evaluation of P. emblica from different habitats.
