Chemo-preventive effect of Angelica sinensis' supercritical extracts on AOM/DSS-induced mouse colorectal carcinoma associated with inflammation.
- Author:
Jing AN
;
Xiao-Ning LI
;
Bo-Chen ZHAO
;
Qiong WANG
;
Yi LAN
;
Qing WU
- Publication Type:Journal Article
- MeSH:
Angelica sinensis;
chemistry;
Animals;
Azoxymethane;
adverse effects;
Colonic Neoplasms;
chemically induced;
genetics;
immunology;
prevention & control;
Colorectal Neoplasms;
chemically induced;
genetics;
immunology;
prevention & control;
Cyclooxygenase 2;
genetics;
metabolism;
Dextran Sulfate;
adverse effects;
Disease Models, Animal;
Drugs, Chinese Herbal;
administration & dosage;
Humans;
Male;
Mice;
Mice, Inbred BALB C;
Proliferating Cell Nuclear Antigen;
genetics;
immunology
- From:
China Journal of Chinese Materia Medica
2014;39(7):1265-1269
- CountryChina
- Language:Chinese
-
Abstract:
To study the chemo-preventive effect of the supercritical extracts from Angelica sinensis (SFE-AS) on induced colorectal carcinoma in mice by using the AOM/DSS-induced male mice colorectal carcinoma model, and discuss its possible action mechanism. Male Balb/c mice were subcutaneously injected with single dose of azoxymethane (AOM, 10 mg x kg(-1) body weight). One week later, they were given 2% dextran sodium sulfate (DSS) in drinking water for 7 days to induce colorectal carcinoma. Each drug group was orally administered with supercritical extracts from Angelica sinensis at 15, 30, 60 mg x kg(-1) until the 17th week. The tumor incidence rate of the SFE-AS group, mice tumor-bearing quantity and tumor-bearing volume of the SFE-AS group were lower than that of the AOM/DSS model control group, which may be related with the significant reduction of PCNA, COX-2, iNOS in the AOM/DSS-induced mouse colorectal carcinoma model associated with inflammation by SFE-AS. According to the results of this study, SFE-AS showed an intervention effect in the incidence and development of AOM/DSS-induced mouse colorectal carcinoma associated with inflammation, and could be further used in chemo-preventive studies on human colorectal carcinoma.
