Inhibitory effect and mechanism of (-)-epigallocatechin-3-gallate on HT29 and HCT-8 colorectal cancer cell lines and expression of HES1 and JAG1.
- Author:
Chun-xia ZHANG
1
;
Shui-ming WANG
;
Hei-ying JIN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Basic Helix-Loop-Helix Transcription Factors; metabolism; Calcium-Binding Proteins; metabolism; Catechin; analogs & derivatives; pharmacology; Cell Line, Tumor; Cell Proliferation; drug effects; Colorectal Neoplasms; pathology; Flow Cytometry; HT29 Cells; Homeodomain Proteins; metabolism; Humans; Intercellular Signaling Peptides and Proteins; metabolism; Jagged-1 Protein; Membrane Proteins; metabolism; Serrate-Jagged Proteins; Transcription Factor HES-1
- From: Chinese Journal of Gastrointestinal Surgery 2011;14(8):636-639
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the inhibitory effect of (-)-epigallocatechin-3-gallate (EGCG) on cancer cells line HCT-8 and HT29 and its influence on the expression of HES1 and JAG1.
METHODSColorectal cancer cells line HCT-8 and HT29 were cultured in vitro and treated with different concentrations of EGCG(10 mg/L, 20 mg/L, 35 mg/L). The inhibition of proliferation was tested by MTT analysis. Influence of EGCG on the cell apoptosis and cell cycle of HCT-8 and HT29 were detected with flow cytometry, and gene expression of HCT-8 and HT29 after EGCG treatment with real-time polymerase chain reaction.
RESULTSEGCG affected the proliferation and apoptosis of HCT-8 and HT29. The inhibition rates of the three different concentrations of EGCG were(28.894±5.076)%, (34.903±1.794)%, and (39.028±0.105)% on HCT-8, and (14.682±4.244)%, (22.429±3.847)%, and (29.840±5.076)% on HT29. EGCG caused G(2)/M phase arrest and M phase transition in HCT-8 cell line, and S phase arrest and G2 phase transition in HT29 cell line. EGCG down-regulated HES1 gene expression in both cell lines, however, the differences were not statistically significant(both P>0.05). EGCG upregulated JAG1 gene expression in both cell lines, however only the difference in HCT-8 was statistically significant(0.201±0.018 vs. 0.440±0.077, P=0.029).
CONCLUSIONSEGCG can significantly inhibit the proliferation of HT29 cells and HCT-8 cells by changing cell cycle and inducing cell apoptosis. The mechanism may be related to the upregulation of JAG1 gene expression.
