Study of tumor necrosis factor-related apoptosis-inducing ligand-induced apoptotic and inflammatory gene expressions in colon cancer cell line.
- Author:
Hong-bin YU
1
;
Wei ZHU
;
Chuang DAI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Apoptosis Regulatory Proteins; genetics; metabolism; Colonic Neoplasms; metabolism; pathology; HCT116 Cells; Humans; Inflammation; Recombinant Proteins; pharmacology; TNF-Related Apoptosis-Inducing Ligand; pharmacology
- From: Chinese Journal of Gastrointestinal Surgery 2011;14(10):803-806
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of recombinant tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) on the expression of apoptosis and inflammatory related genes in human colon cancer cell line HCT-116.
METHODSAfter 24-hour treatment with recombinant human TRAIL protein, the expressions of apoptosis-related genes(Bcl-2, Bad, caspase-3, and caspase-8) and inflammation-related genes(TNF-α, IL-1β, and COX-2) were measured by real-time PCR and appropriate kits in HCT-116.
RESULTSAfter treatments of 10 μg/L and 100 μg/L recombinant human TRAIL proteins, the apoptotic rates of HCT-116 cells were 27.4% and 45.9%, respectively. Expressions of anti-apoptosis gene Bcl-2, pro-apoptosis gene Bad and apoptotic markers caspase-3 and caspase-8 were significantly up-regulated, which was more significant in the group of 100 μg/L treatment(P<0.05). Moreover, after TRAIL treatments, expressions of inflammation-related genes TNF-α, IL-1β, COX-2 were also dramatically increased, and 100 μg/L treatment group showed higher up-regulation(P<0.05).
CONCLUSIONSRecombinant TRAIL protein induces both apoptosis and inflammation of human colon cancer cells.
