Role of CD44(+)/ESA(+) in sorting colonic cancer stem cells.
- Author:
Fang-qin XUE
1
;
Guo-hua YANG
;
Ruo-lei HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Biomarkers, Tumor; Cell Line, Tumor; Colonic Neoplasms; pathology; Flow Cytometry; Humans; Hyaluronan Receptors; Mice; Mice, Inbred NOD; Mice, SCID; Neoplastic Stem Cells; cytology; Sialic Acid Binding Ig-like Lectin 3
- From: Chinese Journal of Gastrointestinal Surgery 2011;14(11):896-898
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo isolate CD133(+)/CD44(+)/ESA(+) subsets cells from SW480 colon cancer cells, and to observe the tumor formation.
METHODCD133(+)/CD44(+)/ESA(+) subsets cells, CD133(-)/CD44(+)/ESA(+) subsets cells and CD133(-)/CD44(-)/ESA(-) subsets cell were sorted by flow cytometry from SW480 colon cancer cells, then three subsets were separately inoculated in five NOD/SCID mice and the growth rates were calculated.
RESULTThe proportion of CD133(-)/CD44(-)/ESA(-), CD133(-)/CD44(+)/ESA(+) and CD133(+)/CD44(+)/ESA(+) subsets cells in SW480 cells were (86.38±10.23)%,(1.26±0.28)% and(0.38±0.07)%. After inoculation, tumor nodules could be formed three days later in CD133(+)/CD44(+)/ESA(+) group, and they could be formed 9 days later in CD133(-)/CD44(+)/ESA(+) group, while they could be formed 15 days later in CD133(-)/CD44(-)/ESA(-) group. Eighteen days later, tumor sizes in three groups were(13.82±5.04) mm(3), (9.25±4.57) mm(3) and (4.76±3.92) mm(3) respectively, and the differences were statistically significant(P<0.05).
CONCLUSIONESA(+)-CD44(+) is one of the surface markers for colonic cancer stem cells, and CD133(+)-CD44(+)-ESA(+) cells are SW480-like cancer stem cells.
