Study on the recombinant adeno-associated virus vector carrying LacZ gene expression in the skeletal muscle.
- Author:
Hong-hua LI
1
;
Su-ming ZHANG
;
Si-yu FANG
;
Chun-lian CHENG
;
Dao-wen WANG
;
Xiao XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Dependovirus; genetics; Embryo, Mammalian; Gene Expression; Genes, Reporter; Genetic Vectors; Humans; Injections, Intramuscular; Kidney; cytology; metabolism; Lac Operon; physiology; Mice; Mice, Inbred C57BL; Muscle, Skeletal; cytology; metabolism; Recombinant Proteins; metabolism; Transfection; methods
- From: Chinese Journal of Medical Genetics 2004;21(6):604-607
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo look for a gene delivery route to the treatment of Duchenne muscular dystrophy(DMD).
METHODSThe recombinant adeno-associated virus vector(rAAV) carrying a LacZ reporter gene was constructed. rAAVLacZ was delivered into the skeletal muscle tissue of C57/BL6 mice by intramuscular injection. Then an intraarterial delivery route was taken to reveal whether rAAVLacZ could transduce muscle tissue.
RESULTS(1) The LacZ gene was efficiently transduced and expressed persisting for 5 months after intramuscular injection. (2) The membrane of muscle and smooth muscle of vessel was widely transduced by intra-arterial delivery rAAVLacZ.
CONCLUSIONThese data provide the evidence that rAAVLacZ can efficiently transduce muscle for a long period. Improving intraarterial gene delivery will be promising means for rAAV-mediated gene therapy for generalized skeletal muscle of DMD.
