Genetic polymorphisms of six short tandem repeat loci in the Han population of Henan province in China.
- Author:
Hui CHEN
1
;
Xiao-wen LI
;
Jian-hua LIAN
;
Guo-ying SONG
;
Xiao-li CHENG
;
Ying HE
;
Hua QI
;
Hua LIU
;
Qian-xian ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Asian Continental Ancestry Group; genetics; China; ethnology; Gene Frequency; Genetic Markers; Genetics, Population; Genotype; Heterozygote; Humans; Paternity; Polymorphism, Genetic; Tandem Repeat Sequences
- From: Chinese Journal of Medical Genetics 2004;21(6):640-642
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the allele frequencies of six short tandem repeats (STR) loci D12S391, D5S818, D18S51, PAHI3, D8S1179, D3S1358 in the Han population of Henan province and to obtain preliminary data.
METHODSDNA was extracted with phenol-chloroform from 140 EDTA-blood specimens of healthy unrelated individuals in Henan population; multiplex PCR technique and PAGE vertical electrophoresis were used to screen the genotype frequencies of six STR systems in Henan population.
RESULTSThe test for Hardy-Weinberg equilibrium revealed that the genotype distribution was correspondent with the expected. The observed heterozygosities of six loci were 0.871, 0.769, 0.871, 0.773, 0.901, 0.722. The calculated discrimination power is 0.9999998, the calculated power of exclusion is 0.99845, the calculated matching probability is 2.39 x 10(-7).
CONCLUSIONAll of the six loci in this study have high power of discrimination and exclusion; they may be very useful genetic markers for individual identification, paternity test and genetics purposes.
