The multiplex analysis of epigenetic markers and genetic markers by post-digestion mutagenically separated PCR.
- Author:
Gui-sen ZHAO
1
;
Dai-xin HUANG
;
Wen-fang FENG
;
Qing-en YANG
Author Information
- Publication Type:Journal Article
- MeSH: DNA Methylation; DNA Restriction Enzymes; metabolism; Genetic Markers; genetics; Humans; Polymerase Chain Reaction; methods; Polymorphism, Single Nucleotide
- From: Chinese Journal of Medical Genetics 2005;22(1):58-60
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a novel method for the multiplex analysis of the methylation and single nucleotide polymorphism (SNP).
METHODSThe imprinted SNP rs220028 was chosen as a model. Genomic DNA, after being digested with methylation sensitive restriction enzyme, were typed by mutagenically separated PCR (MS-PCR). The polymorphism of restriction site was excluded by PCR-RFLP.
RESULTSBy post-digestion MS-PCR, the methylated allele was detected selectively, the maternal origin of which was confirmed by pedigree analysis; A=0.5085, G=0.4915,PIC=0.3749.
CONCLUSIONThe multiplex analysis of methylation markers and SNP can be achieved by post-digestion MS-PCR. The imprinted SNP locus rs220028 is a potentially useful marker in screening Prader-Willi/Angelman syndrome.
