A genetic study on microdeletion of azoospermia factor region on Y chromosome of azoospermia and oligozoospermia patients.
- Author:
Zhi-ming CAI
1
;
Xiao-su XIAO
;
Xiao-yi LIU
;
Yong-qiang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Azoospermia; genetics; Chromosome Aberrations; Chromosomes, Human, Y; genetics; Genetic Loci; Humans; Male; Oligospermia; genetics; Polymerase Chain Reaction; Seminal Plasma Proteins; genetics; Sequence Deletion; Sequence Tagged Sites
- From: Chinese Journal of Medical Genetics 2005;22(1):85-87
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the genetic causes of azoospermia and severe oligozoospermia.
METHODSCytogenetic analysis and multiplex polymerase chain reaction(PCR) analysis were done on the 148 patients with azoospermia and serious oligozoospermia.
RESULTSEleven of the 148(7.4%) cases showed microdeletion of at least one STS. In fifteen STS of AZFa, AZFb,AZFd, AZFc, thirteen STS, eleven STS,two STS and one STS microdeletion were found in each case respectively, including two with 12 STS, five with 5 STS microdeletion.Seven cases had chromosomal morphologic changes(4.7%),four had deletion and one had deletion with translocation of long arm on Y chromosome. One had enlarged region one band two(q12) on long arm of Y chromosome and one had reciprocal translocation of autosomes.
CONCLUSIONThe findings indicated that AZF microdeletion and chromosomal abnormality should be important causes of male infertility.
