Screening for ZNF230 gene mutation and analysis of its correlation with azoospermia.
- Author:
Jing-tao DONG
1
;
Si-zhong ZHANG
;
Yong-xin MA
;
Kai-xuan YANG
;
Ming-kong HUANG
;
Yan SUN
;
Guo-ping HE
;
Ya LI
;
Wei ZHANG
;
Yan PENG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Azoospermia; diagnosis; genetics; Base Sequence; Chromatography, High Pressure Liquid; DNA Mutational Analysis; DNA-Binding Proteins; genetics; Gene Frequency; Genetic Testing; Genotype; Humans; Male; Mutation; Polymerase Chain Reaction; Transcription Factors; genetics; Young Adult
- From: Chinese Journal of Medical Genetics 2005;22(3):258-260
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the possible association between ZNF230 gene and azoospermia.
METHODSScreening for mutation of all 6 exons of ZNF230 gene was performed by denaturing high performance liquid chromatography(DHPLC) in 99 patients with azoospermia and in 115 healthy men as controls.
RESULTSAn A-->G transition at nucleotide 316 in exon 6 was identified. There were significant differences in the distribution profiles of both allele and genotype frequencies between patient group and control group (P < 0.01 and P < 0.05, respectively). In addition,there was a statistically significant difference in the serum follicle stimulating hormone (FSH) level between the patients with GG/GA genotype and those with AA genotype (P < 0.05).
CONCLUSIONZNF230 gene may be associated with azoospermia, and the A316G mutation may be correlated with the serum FSH level.
