Expression of imprinted genes related to Beckwith-Wiedemann syndrome in human oocytes and preimplantation embryos.
- Author:
Wen-jie SHEN
1
;
Fu-qi XING
;
Ling-hong KONG
;
Shi-ling CHEN
;
Hong LI
Author Information
- Publication Type:Journal Article
- MeSH: Beckwith-Wiedemann Syndrome; genetics; Blastocyst; metabolism; Cyclin-Dependent Kinase Inhibitor p57; genetics; Female; Gene Expression Profiling; Genomic Imprinting; genetics; Humans; Nuclear Proteins; genetics; Oocytes; metabolism; Potassium Channels, Voltage-Gated; genetics; Pregnancy; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Medical Genetics 2005;22(3):265-267
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the expression of imprinted genes related to Beckwith-Wiedemann syndrome (BWS) in human oocytes and preimplantation embryos for understanding the relationship between assisted reproductive technology (ART) and BWS.
METHODSUsing nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.
RESULTSTranscripts of P57KIP2 were detected in human oocytes and at all stages of preimplantation embryos. LIT1 was expressed only in stages of 8-cell and blastocyst. Transcripts of TSSC3 could not be detected in human oocytes and preimplantation embryos.
CONCLUSIONTranscripts of P57KIP2 and LIT1, imprinted genes related to BWS, were detected in human preimplantation development; ART might affect the epigenetics of imprinted genes in early embryogenesis.
