Phylogenetic analysis of sporadic hepatitis E virus in Eastern China.
- Author:
Yu-gang XIA
1
;
Yan-ting LI
;
Yi-han LU
;
Hong REN
;
An-qun HU
;
Jian-fu ZHU
;
Xue-cai WANG
;
Qing-wu JING
;
Ying-jie ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Base Sequence; China; epidemiology; Female; Genotype; Hepatitis E; epidemiology; virology; Hepatitis E virus; classification; genetics; isolation & purification; Humans; Male; Middle Aged; Molecular Sequence Data; Phylogeny; RNA, Viral; genetics
- From: Chinese Journal of Epidemiology 2009;30(12):1269-1272
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo understand phylogenetic characteristics of sporadic hepatitis E virus (HEV) in eastern China.
METHODSFour hundred and thirteen sera were collected from sporadic hepatitis E cases in 14 second- or first-class hospitals in Eastern China from 2005 to 2008 and detected with a nested RT-PCR assay. Partial nucleotide sequences of the HEV isolates were determined for phylogenetic analysis with prototype sequences in the GenBank.
RESULTSThe male-to-female sex ratio of the patients was 1.75:1 with 61.5% of them aged 40 - 69 years old. HEV RNA was detected in 140 out of 413(34%)serum samples. Phylogenetic analysis revealed that all the 140 HEV isolates belonged to genotype IV, sharing 77.9% - 88.3%, 80.8% - 90.6%, 73.4% - 85.2% and 91.0% - 95.4% nucleotide sequence identities with prototype I, II, III and IV HEV isolates respectively.
CONCLUSIONIt was evident that genotype IV HEV served as the main causative agent of sporadic HEV infection in Eastern China. However the viral origin and evolution needs further clarification.
