Phylogenetic analysis of sporadic hepatitis E virus in Eastern China
10.3760/cma.j.issn.0254-6450.2009.12.015
- VernacularTitle:华东地区散发性戊型肝炎病毒系统进化分析
- Keywords:
Hepatitis E virus;
Phylogeny;
Genotype
- From:
Chinese Journal of Epidemiology
2009;30(12):1269-1272
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand phylogenetic characteristics of sporadic hepatitis E virus(HEV)in eastern China.Methods Four hundred and thirteen sera were collected from sporadic hepatitis E cases in 14 second-or first-class hospitals in Eastern China from 2005 to 2008 and detected with a nested RT-PCR assay.Partial nucleotide sequences of the HEV isolates were determined for phylogenetic analysis with propotype sequences in the GenBank.Results The male-to-female sex ratio of the patients was 1.75:1 with 61.5% of them aged 40-69 years old.HEV RNA was detected in 140 out of 413(34%)serum samples.Phylogenetic analysis revealed that all the 140 HEV isolates belonged to genotype Ⅳ,sharing 77.9%-88.3%,80.896-90.6%,73.4%-85.2% and 91.0%-95.4% nucleotide sequence identities with prototype Ⅰ,Ⅱ,Ⅲ and Ⅳ HEV isolates respectively.Conclusion It was evident that genotype Ⅳ HEV served as the main causative agent of sporadic HEV infection in Eastern China.However the viral origin and evolution needs further clarification.
