Secretion expression of cholera toxin Bsubunit in food-grading Lactococcus lactis expression system
10.3760/cma.j.issn.0254-6450.2009.12.020
- VernacularTitle:霍乱毒素B亚单位在乳酸乳球菌食品级表达系统中的分泌性表达
- Keywords:
Lactococcus lactis;
Cholera toxin B subunit;
Food-grade vector;
Secretion expression
- From:
Chinese Journal of Epidemiology
2009;30(12):1288-1291
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone and secretion express cholera toxin B subunit(CTB)in food-grading Lactococcus lactis expression systems.Methods ctB fragment that encoding CTB was amplified by polymerase chain reaction(PCR)using the genomic DNA of Vibrio cholera strain 569B as template and was inserted into two secretion expression vector pSQZ and pSQ to construct food-grading expression system L.lactis MBP71/pSQZ-ctB and L.lactis MBP71/pSQ-ctB.The expressed CTB was detected by Western-blot assay.Results The ctB fragment was successfully amplified from Vibrio cholera strain 569B and inserted into two secretion expression vectors pSQZ and pSQ to construct food-grading expression system L.lactis MBP71/pSQZ-ctB and L.lactis MBP71/pSQ-ctB.Western-blot assay demonstrated that CTB was secretion and expressed from L.lactis MBP71 harboring vectors pSQZ-ctB and pSQ-ctB,and the quantity of CTB secreted by L.lactis MBP71/pSQ-ctB was about 2 μg/ml,higher than that of L.lactis MBP71/pSQZ-ctB.Conclusion CTB was successfully secreted and expressed by food-grading L.lactis expression systems.
