Epidemiological investigation on natural infection of different canine breeds with Borna disease virus in Ili, China
10.3760/cma.j.issn.0254-6450.2009.010.002
- VernacularTitle:新疆伊犁地区不同品种犬博尔纳病病毒自然感染调查
- Author:
Qun-Ling ZHAN
1
;
Ying-Ying ZHANG
;
Ming-Ming XU
;
Yong-Bo HU
;
Xiu-Ling WU
;
Jian-Ping YU
;
Xiao CHEN
;
Dan ZHU
;
De-Yu YANG
;
Peng XIE
Author Information
1. 重庆医科大学附属第一医院
- Keywords:
Borna disease virus;
p24 RNA;
Nested reverse transcription-polymerase chain reaction;
TaqMan probe;
Canine
- From:
Chinese Journal of Epidemiology
2009;30(10):993-997
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the epidemioiogical pattern of Borna disease virus (BDV) among different canine breeds in Ili, China, and to analyze its potential phylogeny. Methods BDV p24 RNA fragments were detected from peripheral blood mononuclear cells (PBMCs) of canine by modified nested RT-PCR (nRT-PCR). Possible false positives were excluded by determination of both BDV p40 RNA fragments and PMD19 plasmid standards. Analysis were performed on genetic sequence, homologous comparison, amino acid sequence and phylogeny after p24 positive products were validated. Results BDV p24 RNA fragments were found only in Kazakh Tobet (a shepherd dog) in 8 breeds of 150 cases and their overall positive rate was 11.0% (10/91). Compared with the strain of He/80 from horse and that of S6 from sheep in Germany, the homologous similarities of Kazakh Tobet was 99.2% and 95.7%, and that of amino acid as 100% and 89.3%, respectively. The kinship of Kazakh Tobet was close to He/80 and next to S6. Conclusion There was potential natural BDV infection in Kazakh Tobet in Ili, and its endemic strain was concerned with He/ 80 infecting Ili horse and S6 of German Merino sheep introduced into the region from Germany.
