Complete sequence of the M segment of Amur virus in rodent from China
10.3760/cma.j.issn.0254-6450.2009.010.017
- VernacularTitle:中国宿主动物中Amur汉坦病毒M片段全基因序列分析
- Author:
Wei-Dong GUO
1
;
Wen-Yi ZHANG
;
Jia-Fu JIANG
;
Hai-Yi CHI
;
Wen-Rui WANG
;
Zhong-Yuan WANG
;
Ying JIN
;
Hong YANG
;
Li-Qun FANG
;
Wu-Chun CAO
Author Information
1. 内蒙古自治区疾病预防控制中心
- Keywords:
Hantavirus;
Amur virus;
M segment;
Sequence analysis
- From:
Chinese Journal of Epidemiology
2009;30(10):1051-1054
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the complete sequence of M segment of Amur virus in rodents and to explore their molecular characteristics. Methods Complete M segment of Amur virus in rodent from China was amplified by RT-PCR. The purified PCR product was cloned into pGEM-T Easy vector and then sequenced. Phylogenetic analysis on multiple nucleotide sequences was performed with the Tree PUZZLE and DNAStar software. Results The full-length of its M gene comprised of 3615 nucleotides with one open reading frame (ORF) including 3408 nucleotides and encoding a protein which comprised 1135 amino acids. The ORF was located at bases 41 to 3448. The phylogenetic analysis of JilinAP06 with other hantaviruses revealed that the complete sequence of M segment of JilinAP06 strain was closely related to those Amur viruses such as B78 strain, Liu strain and H5 strain were all from the patients. The complete sequence of M segment of JilinAP06 had only 79.5% identities with the nucleotide sequence of HTNV strain 76-118. Conclusion The complete sequence on M segment of Amur virus in rodent was first time identified in this country.
