Identification and genetic characteristics on the bacteria isolate from a case of human Brucellosis in Guizhou province.
- Author:
Shi-jun LI
1
;
Yue WANG
;
Ding-ming WANG
;
Ke-cheng TIAN
;
Ying LIU
;
Qing MA
;
Zhao-bin LIU
;
Xiao-Jun GONG
;
Guang-Peng TANG
;
Gui-Chun CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Bacterial Typing Techniques; methods; Brucella; classification; genetics; isolation & purification; Brucellosis; epidemiology; microbiology; China; epidemiology; DNA, Bacterial; genetics; Genotype; Humans; Male
- From: Chinese Journal of Epidemiology 2013;34(7):717-720
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEA suspected Brucella (B.) strain(GZZA), isolated from a case of anti-Brucella antibody positive patient was identified and its' genetic characteristics was analyzed, to provide etiologic basis for the confirmation of patient in Guizhou province.
METHODSConventional methods and polymerase chain reaction(PCR)were used to identify the bacteria strain, with genetic characteristics analyzed by MLVA-16.
RESULTSThe bacteria strain was identified as B. melitensis biovar 3 under the conventional and PCR methods. Results from the MLVA-16 analysis indicated that the bacteria strain was closely clustered with B. melitensis biovar 3, and differences of repeated numbers at VNTR loci bruce42, bruce04, bruce09 and bruce16 were also displayed.
CONCLUSIONBoth traditional and molecular methods to identify one bacteria strain isolated from the human patient as B. melitensis biovar 3 and the genetic characteristics of the strain was closely related to that of B. melitensis biovar 3. Differences of repeated numbers at part of VNTR loci were also showed. The results of this study provided etiologic evidences for the confirmation of Brucella infection of the patient, also providing scientific basis for the control and prevention of Brucellosis in Guizhou province.
