Study on the role of western blot band profile for the detection of recent HIV infection.
- Author:
Ji-bao WANG
1
;
Na ZHANG
2
;
Hai-ying YU
3
;
Yan-ling LI
1
;
Xing DUAN
1
;
Hao YAN
3
;
Jin YANG
1
;
Song DUAN
4
;
Yan JIANG
5
Author Information
- Publication Type:Journal Article
- MeSH: Acquired Immunodeficiency Syndrome; blood; diagnosis; epidemiology; Adolescent; Adult; Aged; Aged, 80 and over; Blotting, Western; Child; Child, Preschool; China; Female; HIV Seropositivity; HIV-1; immunology; Humans; Male; Middle Aged
- From: Chinese Journal of Epidemiology 2013;34(10):998-1002
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo obtain information on HIV disease progression through western blot (WB) band profile.
METHODS1466 available serum samples of newly reported HIV/AIDS cases, confirmed by WB from Dehong prefecture of Yunnan province in 2011, were tested, using the BED HIV Incidence Capture Enzyme Immunoassay (BED-CEIA) and Limiting Antigen Avidity Enzyme Immunoassay (LAg-Avidity EIA), respectively. Differences of WB band profiled between recent infections and established infections were then compared.
RESULTSInfections defined as 'recent' showed lower mean antibody intensities to p17, p24, p31, gp41, p51, p55, p66, gp120 and gp160 in WB when compared to those established infections with statistical difference(P < 0.05).
CONCLUSIONThese WB band profiles which were considered as 'recent' infections would include factors as:lack of antibody reactivity to gp41 antigen, with intensity scores of all bands lower than 13, and those intensity scores of antibody to env protein lower than 5 or to pol protein lower than 4.
