Application of PulseNet China Database in tracking and warning 4 cholerae outbreaks in Anhui province, in 2012.

Shou-kui HU; Tian-li Zheng; Wan-fu HU; Jian-jun Wang; Wei LI; Zhi-gang Cui; Hai-jian Zhou; Bo Pang; Biao Kan

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Application of PulseNet China Database in tracking and warning 4 cholerae outbreaks in Anhui province, in 2012.

Author: Shou-kui HU ¹ ; Tian-li ZHENG ² ; Wan-fu HU ¹ ; Jian-jun WANG ³ ; Wei LI ⁴ ; Zhi-gang CUI ⁴ ; Hai-jian ZHOU ⁴ ; Bo PANG ⁵ ; Biao KAN ⁴
Author Information

1. Anhui Center for Disease Control and Prevention, Hefei 230061, China.
2. College of Public Health, Sichuan University.
3. Anhui Center for Disease Control and Prevention, Hefei 230061, China. Email: wjj@ahcdc.com.cn.
4. State Key Laboratory for Infectious Disease Control and Prevention,Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
5. State Key Laboratory for Infectious Disease Control and Prevention, Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention.
Publication Type:Journal Article
MeSH: Animals; Bacterial Typing Techniques; methods; China; epidemiology; Cholera; epidemiology; prevention & control; Cluster Analysis; DNA, Bacterial; Databases, Factual; Electrophoresis, Gel, Pulsed-Field; methods; Humans; Polymerase Chain Reaction; Vibrio cholerae; genetics
From: Chinese Journal of Epidemiology 2013;34(10):1003-1005
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo track the source of infection regarding 4 Cholerae outbreaks in Anhui province in 2012 through the application of PulseNet China Database (PNCD).
METHODSCholerae virulence genes were amplified by PCR and typed by pulse field gel electrophoresis(PFGE). Results from electrophoresis were cluster-analyzed by BioNumericsV4.0 software and compared with PNCD.
RESULTSVirulence gene CT and TCP of the tested vibrio cholera showed both positive. Homology of the strains from four cholera outbreaks was more than 98%, based on the homologous and cluster analysis through enzyme digested PFGE electrophoresis. Those strains were highly homologous with the cholera epidemic strains identified in Hunan, Sichuan,Zhejiang, Shanghai and Hubei by PNCD, with the homology as 100% .
CONCLUSIONFour cholera outbreaks in Anhui province, 2012 were highly correlated with the outbreaks occurring in Hunan and Sichuan during the same time period, indicating that PNCD could effectively and quickly tracking down the source of infection on the cholera outbreaks and providing early warning of the situation.