Comparative studies on different methods for laboratory diagnosis of pertussis
10.3760/cma.j.issn.0254-6450.2013.10.016
- VernacularTitle:百日咳实验室诊断方法的应用分析与比较
- Author:
Zengguo WANG
1
;
Yang YANG
;
Ying LIU
;
Xiao-Guai LIU
;
Yan-Li XI
;
Ling-Xia LEI
;
Ya-Rong LI
Author Information
1. 710054,西安市疾病预防控制中心
- Keywords:
Pertussis;
Enzyme linked immunosorbent assay;
Polymerase chain reaction
- From:
Chinese Journal of Epidemiology
2013;34(10):1010-1012
- CountryChina
- Language:Chinese
-
Abstract:
Objective To confirm the clinically suspected pertussis cases (＜ 1 years old) through laboratory methods.Methods From December,2011 to December,2012,patients with clinically suspected pertussis from Xi'an Children's Hospital were sampled,with their nasopharyngeal swabs collected,blood samples cultured and pertussis toxin IgG detected by PCR.Results were analyzed,using SPSS 16.0 software.Results 100 out of the 148 cases were laboratorially confirmed.3,88 and 34 cases were positive,through culture,PCR or pertussis toxin IgG respectively.22 cases were both PCR and pertussis toxin IgG positive.There were significant differences between the results of IS481 PCR,days from the onset of symptoms (P＜0.01) and results of PT-IgG with the days from onset of symptoms (P＜0.01).Conclusion Since the sensitivity of culture on pertussis was low,diagnosis on the disease should be linked to the results from PCR,PT-IgG and the days from onset of symptoms.
