Study on seed quality test and quality standard of Lonicera macranthoides.

Ying Zhang; Jin XU; Long-yun LI; Guang-lin Cui; Yue-hui She

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Study on seed quality test and quality standard of Lonicera macranthoides.

Author: Ying ZHANG ¹ ; Jin XU ¹ ; Long-Yun LI ¹ ; Guang-Lin CUI ¹ ; Yue-Hui SHE ²
Author Information

1. Chongqing Academy of Chinese Material Medica, Chongqing Research Center of Techniques and Engineering for Chinese Material Medica Fine Varierty Breeding and Evaluation, Chongqing 400065, China.
2. Sichuan Agricultural Universitry, Ministry of Agriculture, Chengdu 611130, China.
Publication Type:Journal Article
Keywords: Lonicera macranthoides; quality classification standard; quality test methods; seed
From: China Journal of Chinese Materia Medica 2016;41(8):1439-1445
CountryChina
Language:Chinese
Abstract: Referring to the rules for agricultural seed testing (GB/T 3543-1995) issued by China, the test of sampling, purity, thousand seed weight, moisture, viability, relative conductivity and germination rate had been studied for seed quality test methods of Lonicera macranthoides. The seed quality from 38 different collection areas was measured to establish quality classification standard by K-means clustering. The results showed that at least 7.5 g seeds should be sampled, and passed 20-mesh sieve for purity analysis.The 500-seed method used to measure thousand seed weight. The moisture was determined by crushed seeds dried in high temperature (130±2) ℃ for 3 h.The viability determined by 25 ℃ 0.1% TTC stained 5h in dark. 1.0 g seeds soaked in 50 ml ultra pure water in 25 ℃ for 12 hours to determine the relative conductivity. The seed by 4 ℃stratification for 80 days were cultured on paper at 15 ℃. Quality of the seeds from different areas was divided into three grades. The primary seed quality classification standard was established.The I grade and II grade were recommend use in production.