Construction of an allogenic chimeric mouse model for the study of the behaviors of donor stem cells in vivo.
- Author:
Mo-lin WANG
1
;
Jing-bin YAN
;
Yan-ping XIAO
;
Shu-zhen HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Flow Cytometry; Hematopoietic Stem Cell Transplantation; In Situ Hybridization, Fluorescence; Mice; Models, Animal; Polymerase Chain Reaction; Transplantation Chimera; Transplantation, Homologous
- From: Chinese Medical Journal 2005;118(17):1444-1450
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDIt is essential to establish an animal model for the elucidation of the biological behaviors of stem cells in vivo. We constructed a chimeric animal model by in utero transplantation for investigation of stem cell transplantation.
METHODSThis chimerism was achieved by injecting the stem cells derived from the bone marrow of green fluorescence protein (GFP)-transgenic mice into fetal mice at 13.5 days of gestation. Several methods such as polymerase chain reaction (PCR), real-time PCR, fluorescence-assisted cell sorting (FACS) and fluorescence in situ hybridization (FISH) were used for the observation of donor cells.
RESULTSUnder a fluorescence microscope, we observed the GFP cells of donor-origin in a recipient. PCR, FACS analysis and FISH indicated chimerism at various intervals. Real-time PCR indicated that some donor cells existed in chimera for more than 6 months.
CONCLUSIONSAllogenic stem cells may exist in recipients for a long time and this allogenic animal model provides a useful tool for studying the behavior of hematopoietic stem cells and also offers an effective model system for the study of stem cells.
