The effect of gamma-ray irradiation at low temperature on the stability and cytocompatibility of collagen membrane in vitro.
- Author:
Xiaoyan LIN
1
;
Min TANG
;
Xingdong ZHANG
Author Information
1. School of Materials, Southwest University of Science and Technology, Mianyang 621002, China. x8ylin-2004@136.com
- Publication Type:Journal Article
- MeSH:
Animals;
Cattle;
Collagen;
radiation effects;
Dose-Response Relationship, Radiation;
Gamma Rays;
In Vitro Techniques;
Materials Testing;
Membranes, Artificial;
Mice
- From:
Journal of Biomedical Engineering
2006;23(4):822-825
- CountryChina
- Language:Chinese
-
Abstract:
Membranes prepared from bovine skin collagen were exposed to 15, 25, 35 KGy gamma-radiation respectively at low temperature. Radiation dose rate of about 22 KGy/h was used. The stability of the membranes was evaluated by measuring resistance of collagen membranes to collagenase digestion. Infrared spectra analyses of collagen films were performed in order to explore possible mechanisms of irradiation modification of collagen membranes. The results revealed that the degree of cross-linking and stability of collagen membranes after gamma-irradiation were improved. The MTT assay and SEM observation of the morphology of L929 mouse fibroblast cells which directly cultured on the collagen membranes were employed to evaluate the cytocompatibility of collagen membranes treated by gamma-ray radiation. In the range of 0 to approximately 25 KGy irradiation dose, no significant difference in cytocompatibility of collagen membranes irradiated by gamma-ray was observed. However, when the irradiation dose was over 25 KGy, the cytocompatibility of collagen membranes was influenced by gamma-radiation to some degree.
