Strategies for antibody immobilization during the fabrication of antibody microarrays.
- Author:
Lu ZHANG
1
;
Bicheng LIU
Author Information
1. Institute of Nephrology, Zhongda Hospital, Southeast University, Nanjing 210009, China.
- Publication Type:Journal Article
- MeSH:
Adsorption;
Antibodies, Monoclonal;
chemistry;
metabolism;
Protein Array Analysis;
methods;
Protein Binding
- From:
Journal of Biomedical Engineering
2006;23(4):907-910
- CountryChina
- Language:Chinese
-
Abstract:
As one of the proteomic technologies, antibody microarrays can provide a powerful tool for the high-throughput parallel analysis of thousands of proteins in a single experiment. Because antibodies are chemically and structurally complex; immobilizing antibody on solid phase surface of substrates has been a key step during the development of antibody microarrays. This review will focus on the current strategies for antibody immobilization and discuss the advantages and disadvantages of these methods.
