Analysis of FT-IR-ATR spectra of serum proteins adsorbed on carbonaceous materials.
- Author:
Bogang LI
1
;
Juanjuan NA
;
Jie YIN
;
Guangfu YIN
Author Information
1. College of Materials Science and Engineering, Sichuan University, Chengdu 610065, China.
- Publication Type:Journal Article
- MeSH:
Adsorption;
Blood Proteins;
metabolism;
Diamond;
chemistry;
Graphite;
chemistry;
Humans;
Hydrogen Bonding;
In Vitro Techniques;
Membranes, Artificial;
Spectroscopy, Near-Infrared
- From:
Journal of Biomedical Engineering
2006;23(5):1052-1055
- CountryChina
- Language:Chinese
-
Abstract:
To clarify the reason causing difference of serum proteins adsorbability on different carbonaceous materials, FT-IR-ATR spectra of human serum albumin (HSA) and human serum fibrinogen(HFG) before and after adsorbing on diamond like carbon film (DLC),diamond film (DF) and graphite were analyzed. It has been shown that there are hydrogen bond because of -NH at the interfaces of HSA-DLC, HFG-DF and HFG-graphite. Based on the results, earlier research conclusion that the adsorbability of HSA on DLC higher than that on DF and graphite, but on DF and graphite the adsorption of HFG takes precedence can be explained rationally.
