Encapsulating hepatocytes with chitosan in physiological conditions.
- Author:
Jianhang ZHU
1
;
Bao ZHANG
;
Xiluan YAN
;
Xuejun LAO
;
Hanry YU
Author Information
1. Department of Chemical Engineering, Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330029, China. envzjh@ncu.edu.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Capsules;
Cell Culture Techniques;
methods;
Cells, Cultured;
Chitosan;
chemistry;
pharmacology;
Hepatocytes;
cytology;
drug effects;
Male;
Methacrylates;
Methylmethacrylate;
Polymers;
Rats;
Rats, Wistar;
Tissue Engineering;
methods
- From:
Journal of Biomedical Engineering
2006;23(5):1070-1074
- CountryChina
- Language:Chinese
-
Abstract:
Prepared from 15.3% N-acetylated chitosan (FNC), half N-acetylated chitosan (HNC) possesses a good solubility in a weak basic solution, guaranteeing the formation of microcapsules by the coacervating reaction between HNC and methacrylic acid (MAA)-hydroxyethyl methacrylate (HEMA)-methyl methacrylate (MMA) (MAA-HEMA-MMA) terpolymer under physiological conditions. When hepatocytes were encapsulated in such 3-dimensional microenvironment, as compared to monolayer culture, cell functions, including P450 activity, urea production and albumin release, were well supported. The prepared microcapsules have good mechanical stability and permeability.
