Differential responses to UVB irradiation in human keratinocytes and epidermoid carcinoma cells.
- Author:
Mei Juan ZHOU
;
Li ZHENG
;
Ling GUO
;
Wei Ling LIU
;
Chao LV
;
Li Hong JIANG
;
Cheng Shan OU
;
Zhen Hua DING
- Publication Type:Letter
- MeSH: Antioxidants; pharmacology; Apoptosis; radiation effects; Carcinoma, Squamous Cell; Cell Cycle Checkpoints; Cell Line, Tumor; Cell Survival; drug effects; radiation effects; Gene Expression Regulation, Neoplastic; Glucosides; pharmacology; Humans; Keratinocytes; radiation effects; Phenols; pharmacology; Ultraviolet Rays
- From: Biomedical and Environmental Sciences 2012;25(5):583-589
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo examine UVB-induced responses in normal human keratinocytes (HaCaT) and epidermoid carcinoma cells (A431) at the cellular and molecular level, and investigated the protective effect of salidroside.
METHODSCells irradiated by UVB at various dosage and their viability was assessed by MTT assays, cell cycle was analysed by flow cytometry. The expression of NF-κB, BCL-2, and CDK6 after 50 J/m(2) UVB irradiation were detected by RT-PCR and western blotting.
RESULTSOur results confirmed greater tolerance of A341 cells to UVB-induced damage such as cell viability and cell cycle arrest, which was accompanied by differential expression changes in NF-κB, BCL-2, and CDK6. UVB exposure resulted in HaCaT cells undergoing G(1)-S phase arrest. When treated with salidroside, HaCaT survival was significantly enhanced following exposure to UVB, suggesting great therapeutic potential for this compound.
CONCLUSIONTaken together, our study suggests that A431 respond differently to UVB than normal HaCaT cells, and supports a role for NF-κB, CDK6, and BCL-2 in UVB-induced cell G(1)-S phase arrest. Furthermore, salidroside can effectively protect HaCaT from UVB irradiation.