In vitro protein expression profile of Campylobacter jejuni strain NCTC11168 by two-dimensional gel electrophoresis and mass spectrometry.
- Author:
Mao Jun ZHANG
1
;
Yi Xin GU
;
Xiao DI
;
Fei ZHAO
;
Yuan Hai YOU
;
Fan Liang MENG
;
Jian Zhong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Proteins; genetics; metabolism; Campylobacter jejuni; classification; genetics; metabolism; Electrophoresis, Gel, Two-Dimensional; methods; Gene Expression Regulation, Bacterial; physiology; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; methods; Transcriptome
- From: Biomedical and Environmental Sciences 2013;26(1):48-53
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the protein expression profiles of the major food-borne pathogen Campylobacter jejuni NCTC11168.
METHODSMembrane and soluble cellular proteins were extracted from the genome-sequenced C. jejuni strain NCTC11168. Protein expression profiles were determined using two-dimensional gel electrophoresis (2-DE). All the detected spots on the 2-DE map were subjected to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF) analysis.
RESULTSA total of 537 and 333 spots were detected from the whole cell and membrane-associated proteins of C. jejuni NCTC11168 cultured on Columbia agar medium at 42 °C by 2-DE and Coomassie Brilliant Blue staining, respectively. Analyses of whole cell and membrane-associated proteins included 399 and 133 spots, respectively, which included 182 and 53 functional proteins identified by MALDI-TOF/TOF analysis.
CONCLUSIONThe comprehensive expression protein profiles of C. jeuni NCTC11168 obtained in this study will be useful for elucidating the roles of these proteins in further pathogenesis investigation.
