Isolation and characterization of an algicidal bacterium indigenous to lake Taihu with a red pigment able to lyse microcystis aeruginosa.
- Author:
Fei YANG
1
;
Hai Yan WEI
;
Xiao Qin LI
;
Yun Hui LI
;
Xiao Bo LI
;
Li Hong YIN
;
Yue Pu PU
Author Information
- Publication Type:Journal Article
- Keywords: Algae-lysing; Algicidal bacteria; Harmful algal blooms (HABs); Microcystis aeruginosa; Serratia marcescens
- MeSH: Anti-Bacterial Agents; pharmacology; Bacteria; classification; genetics; metabolism; Lakes; Microcystis; growth & development; Phylogeny
- From: Biomedical and Environmental Sciences 2013;26(2):148-154
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo isolate and characterize indigenous algicidal bacteria and their algae-lysing compounds active against Microcystis aeruginosa, strains TH1, TH2, and FACHB 905.
METHODSThe bacteria were identified using the Biolog automated microbial identification system and 16S rDNA sequence analysis. The algae-lysing compounds were isolated and purified by silica gel column chromatography and reverse-phase high performance liquid chromatography. Their structures were confirmed by Nuclear Magnetic Resonance (NMR) and Fourier Transform Infrared (FT-IR) spectroscopy. Algae-lysing activity was observed using microscopy.
RESULTSThe algae-lysing bacterium LTH-2 isolated from Lake Taihu was identified as Serratia marcescens. Strain LTH-2 secreted a red pigment identified as prodigiosin (C20H25N3O), which showed strong lytic activity with algal strains M. aeruginosa TH1, TH2, and FACHB 905 in a concentration-dependent manner. The 50% inhibitory concentration (IC50) of prodigiosin with the algal strains was 4.8 (± 0.4)× 10⁻² μg/mL, 8.9 (± 1.1)× 10⁻² μg/mL, and 1.7 (± 0.1)× 10⁻¹ μg/mL in 24 h, respectively.
CONCLUSIONThe bacterium LTH-2 and its pigment had strong Microcystis-lysing activity probably related to damage of cell membranes. The bacterium LTH-2 and its red pigment are potentially useful for regulating blooms of harmful M. aeruginosa.
