Single and combined effects of estrone and 17β-estradiol on male goldfish.

Zhen Hua Yan; Guang Hua LU; Xiao Fan Yang

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Single and combined effects of estrone and 17β-estradiol on male goldfish.

Author: Zhen Hua YAN ¹ ; Guang Hua LU ; Xiao Fan YANG
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1. Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, Jiangsu, China.
Publication Type:Journal Article
Keywords: Carassius auratus; DNA damage; EROD activity; Vitellogenin
MeSH: Animals; Cytochrome P-450 CYP1A1; metabolism; DNA Damage; drug effects; Drug Combinations; Estradiol; pharmacology; Estrone; pharmacology; Goldfish; Gonads; drug effects; metabolism; Male; Vitellogenins; blood
From: Biomedical and Environmental Sciences 2013;26(3):176-184
CountryChina
Language:English
Abstract:
OBJECTIVETo assess the single and combined effects of estrone (E1) and 17β-estradiol (E2) on goldfish (Carassius auratus).
METHODSBatch tests were conducted. Serum levels of vitellogenin (VTG) and E2, gonadosomatic indices (GSI), gonadal DNA damage and liver 7-ethoxyresorufin-O-deethylase (EROD) activity were measured after exposure for 14 days.
RESULTSThe VTG level increased significantly in a concentration-dependent manner. The serum E2 level was significantly higher and the GSI level was significantly lower in goldfish after exposed to the 3 drugs. DNA damage occurred in treated samples and EROD activity was significantly suppressed 7 days after exposure. The joint effect of E1 and E2 was additive with regard to VTG induction.
CONCLUSIONThe results of our study highlight a series of effects of steroidal estrogens on goldfish. Further study is needed to confirm their effect as a whole.