A study of the technique of western blot for diagnosis of lyme disease caused by Borrelia afzelii in China.
- Author:
Zhi Yun LIU
1
;
Qin HAO
;
Xue Xia HOU
;
Yi JIANG
;
Zhen GENG
;
Yi Mou WU
;
Kang Lin WAN
Author Information
- Publication Type:Journal Article
- Keywords: Borrelia afzelii; Diagnostic method; Lyme disease; Western blot
- MeSH: Blotting, Western; methods; Borrelia burgdorferi Group; pathogenicity; China; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Humans; Lyme Disease; diagnosis; microbiology
- From: Biomedical and Environmental Sciences 2013;26(3):190-200
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the technique of Western blot for the diagnosis of Lyme disease caused by Borrelia afzelii in China and to establish the standard criteria by operational procedure.
METHODSFP1, which is the representative strain of B. afzelii in China, was analyzed by SDS-PAGE, electro transfer and immunoblotting assays. The molecular weights of the protein bands of FP1 were analyzed by Gel-Pro analysis software. In a study using 451 serum samples (159 patients with Lyme disease and 292 controls), all observed bands were recorded. The accuracy of the WB as a diagnostic test was established by using the ROC curve and Youden index.
RESULTSCriteria for a positive diagnosis of Lyme disease were established as at least one band of P83/100, P58, P39, OspB, OspA, P30, P28, OspC, P17, and P14 in the IgG test and at least one band of P83/100, P58, P39, OspA, P30, P28, OspC, P17, and P41 in the IgM test. For IgG criteria, the sensitivity, specificity and Youden index were 69.8%, 98.3%, and 0.681, respectively; for IgM criteria, the sensitivity, specificity and Youden index were 47%, 94.2%, and 0.412, respectively.
CONCLUSIONEstablishment of WB criteria for B. afzelii is important in validating the diagnostic assays for Lyme disease in China.
