Impact of sub-chronic aluminium-maltolate exposure on catabolism of amyloid precursor protein in rats.
- Author:
Rui Feng LIANG
1
;
Wei Qing LI
;
Hong WANG
;
Jun Xia WANG
;
Qiao NIU
Author Information
- Publication Type:Journal Article
- Keywords: Aluminium-maltolate; Amyloid precursor protein; Rat; β-amyloid protein
- MeSH: Amyloidogenic Proteins; genetics; metabolism; Animals; Drug Administration Schedule; Environmental Pollutants; administration & dosage; toxicity; Gene Expression Regulation; drug effects; Male; Organometallic Compounds; administration & dosage; toxicity; Pyrones; administration & dosage; toxicity; Random Allocation; Rats; Rats, Sprague-Dawley
- From: Biomedical and Environmental Sciences 2013;26(6):445-452
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the impact of sub-chronic Aluminium-maltolate [Al(mal)3] exposure on the catabolism of amyloid precursor protein (APP) in rats.
METHODSForty adult male Sprague-Dawley (SD) rats were randomly divided into five groups: the control group, the maltolate group (7.56 mg/kg BW), and the Al(mal)3 groups (0.27, 0.54, and 1.08 mg/kg BW, respectively). Control rats were administered with 0.9% normal saline through intraperitoneal (i.p.) injection. Maltolate and Al(mal)3 were administered to the rats also through i.p. injections. Administration was conducted daily for two months. Rat neural behavior was examined using open field tests (OFT). And the protein expressions and their mRNAs transcription related with APP catabolism were studied using enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR).
RESULTSThe expressions of APP, β-site APP cleaving enzyme 1 (BACE1) and presenilin-1 (PS1) proteins and their mRNAs transcription increased gradually with the increase of Al(mal)3 doses (P<0.05). The enzyme activity of BACE1 in the 0.54 and 1.08 mg/kg Al(mal)3 groups increased significantly (P<0.05). The expression of β-amyloid protein (Aβ) 1-40 gradually decreased while the protein expression of Aβ1-42 increased gradually with the increase of Al(mal)3 doses (P<0.05).
CONCLUSIONResult from our study suggested that one of the possible mechanisms that Al(mal)3 can cause neurotoxicity is that Al(mal)3 can increase the generation of Aβ1-42 by facilitating the expressions of APP, β-, and γ-secretase.
