Experimental study on effect of gastrodia in inducing the differentiation of mesenchymal stem cells into neuron-like cells.
- Author:
Xiao-xian DONG
1
;
Jin-bao LIU
;
Yan-xiang DONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Bone Marrow Cells; cytology; Cell Differentiation; drug effects; Cells, Cultured; Drugs, Chinese Herbal; pharmacology; Gastrodia; Glial Fibrillary Acidic Protein; metabolism; Intermediate Filament Proteins; metabolism; Male; Mesenchymal Stromal Cells; cytology; Nerve Tissue Proteins; metabolism; Nestin; Neurons; cytology; Phosphopyruvate Hydratase; metabolism; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Integrated Traditional and Western Medicine 2004;24(1):51-54
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of gastrodia in extracorporeal oriented inducing the differentiation of mesenchymal stem cells into neuron-like cells.
METHODSMesenchymal stem cells were separated from bone marrow of rats by wall sticking method, amplifying cultured in vitro, and differentiated into neuron-like cells by oriented induction with gastrodia. The morphology of cells was observed under light microscopy, neuro-specific enolase (NSE), nestin and glial fibrillary acidic protein (GFAP) were detected by immunocytochemistry.
RESULTSRats mesenchymal stem cells could be separated and amplified in vitro. After being induced by gastrodia for 2 hrs, most of the cells would be differentiated into meuron-like cells, revealing cytodendrite. By immunochemical staining, cells showed positive of NSE, nestin, and negative of GFAP.
CONCLUSIONRats' mesenchymal stem cells could be induced to differentiate into neuron-like cells.
