Effect of buyang huanwu decoction drug serum on expression of p53 and p21 genes in cultured rat's cerebral cortical neuron after hypoxia in vitro.
- Author:
Hong-da QU
1
;
Li TONG
;
Jian-gang SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Cell Hypoxia; Cells, Cultured; Cerebral Cortex; pathology; Drugs, Chinese Herbal; pharmacology; Female; Male; Neurons; pathology; Oncogene Protein p21(ras); biosynthesis; genetics; Rats; Rats, Wistar; Serum; Tumor Suppressor Protein p53; biosynthesis; genetics
- From: Chinese Journal of Integrated Traditional and Western Medicine 2004;24(2):133-135
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of Buyang Huanwu decoction (BHD) drug serum on rat's in vitro cultured cerebral cortical neuron apoptosis induced by hypoxia, and on the expression of p53 and p21 genes in hypoxia process.
METHODSThe model of hypoxia neuron apoptosis was established adopting Daniel method and treated with BHD drug serum. The neuron apoptosis rate was determined by flow cytometry with propidium iodide staining, the p53 and p21 gene expression was tested by immunohistochemical method with flow cytometry.
RESULTSBHD could significantly inhibit the neuron apoptosis induced by hypoxia and down-regulate the expressions of p53 and p21 genes.
CONCLUSIONBHD shows inhibition on neuron hypoxia apoptosis and down-regulating of the p53 and p21 gene expression is one of its mechanisms.
