Effect of jingui shenqi pill on pituitary adrenocorticotropic hormone gene expression in shen-yang deficiency rats.
- Author:
Xiao-wei ZHENG
1
;
Su-zhen BAO
;
Ming-zhe LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adrenocorticotropic Hormone; biosynthesis; genetics; Animals; Drugs, Chinese Herbal; pharmacology; Hypothalamo-Hypophyseal System; Kidney Diseases; genetics; metabolism; Medicine, Chinese Traditional; Pituitary Gland; metabolism; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Sprague-Dawley; Yang Deficiency; genetics; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2004;24(3):238-240
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of Jingui Shenqi pill (JGSQP) with various concentrations at different time points on pituitary adrencorticotropic hormone (ACTH) gene expression level in Shen-Yang deficiency rats.
METHODSThe Shen-Yang deficiency rats were randomly divided into the model control group and the high, medium and low dosage of JGSQP groups. Reverse transcriptase polymerase chain reaction was used to observe the effect of JGSQP on the ACTH mRNA of pituitary tissue in rats treated at different time points (10 d, 20 d, 30 d).
RESULTSAs compared with that in the model group, the ACTH gene expression level was significantly higher in the high dose JGSQP group (P < 0.05), and the increment in the medium dosage group was significantly higher in comparing with that in the high and low dosage groups (P < 0.05 or P < 0.01).
CONCLUSIONThrough up-regulation on ACTH gene expression is possibly one of the mechanisms of JGSQP in treating Shen-Yang deficiency.
