Comparison of the biodistribution of four contrast agents in nude mice bearing NCI-H358 human lung cancer and evaluation of their value in diagnostic imaging.
- Author:
Jian-yang ZHANG
1
;
Jue FENG
;
Hong-mei LI
;
Xiao-dong LI
;
Hong-li JIA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma, Non-Small-Cell Lung; diagnostic imaging; metabolism; pathology; Cell Line, Tumor; Contrast Media; pharmacokinetics; Female; Fluorodeoxyglucose F18; pharmacokinetics; Humans; Lung Neoplasms; diagnostic imaging; metabolism; pathology; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Transplantation; Oligopeptides; pharmacokinetics; Organotechnetium Compounds; pharmacokinetics; Radiopharmaceuticals; pharmacokinetics; Technetium Tc 99m Sestamibi; pharmacokinetics; Thiocarbamates; pharmacokinetics; Tissue Distribution; Tomography, Emission-Computed, Single-Photon; methods
- From: Chinese Journal of Oncology 2011;33(7):504-507
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the uptake of four contrast agents: (99)Tc(m)-RGD-4CK, (99)Tc(m)-N(NOET)(2), (99)Tc(m)-MIBI and (18)F-FDG in Bal B/c nude mice bearing human non-small cell lung cancer NCI-H358 and evaluate their diagnostic value in low-metabolic lung cancer.
METHODSHuman bronchioloalveolar carcinoma NCI-H358 cells were subcutaneously inoculated in Bal B/c nude mice to establish mouse models bearing human lung cancer. Twenty tumor-bearing nude mice were given injection of the four contrast agent, respectively, 5 mice in each group. SPECT imaging and biodistribution of the 4 tracers in the tumor-bearing nude mice were performed. The ratios of tumor to non-tumor (T/NT) of the tracers were compared.
RESULTSThe results from semi-quantification of the planar image and assessment of biodistribution showed that tumor to contralateral muscle activity ratios (T/NT) of the four tracers had statistically significant difference between each two of the four tracer groups of tumor-bearing mice (P < 0.001), with a highest value of T/NT ratio in the (99)Tc(m)-RGD-4CK group.
CONCLUSIONSNCI-H358 tumors show a higher uptake of (99)Tc(m)-RGD-4CK than (18)F-FDG. It suggests that when diagnosing a well-differentiated lung cancer such as bronchioloalveolar carcinoma, the contrast agent (99)Tc(m)-RGD-4CK may be more sensitive than (18)F-FDG, and it may become a promising contrast agent in tumor imaging diagnosis.
