Influence of knock-down of miR-21 expression on the radiosensitivity of glioma SHG-44 cells.
- Author:
Ju-ying ZHOU
1
;
Chong ZHOU
;
Li-li WANG
;
Xiao-ting XU
;
Song-bing QIN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Caspase 3; metabolism; Cell Line, Tumor; Gene Expression Regulation, Neoplastic; Gene Knockdown Techniques; Glioma; genetics; metabolism; pathology; Humans; MicroRNAs; genetics; metabolism; Oligonucleotides, Antisense; genetics; metabolism; Radiation Tolerance; Transfection
- From: Chinese Journal of Oncology 2011;33(10):747-751
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the influence of knock-down of miR-21 expression on the radiosensitivity of radioresistant glioma SHG-44 cells and its mechanism.
METHODSRadioresistant cell line SHG-44(R) cells were established from radiosensitive parental SHG-44 glioma cells. Then the expression levels of miR-21 in SHG-44(R) and SHG-44 were determined by quantitative real-time PCR. The effect of miR-21 on the radiosensitivity was assessed in SHG-44(R) with miR-21 inhibitor to decrease the miR-21 expression.
RESULTSmiR-21 was up-regulated 1.49-fold in SHG-44(R) cells relative to the SHG-44 cells. But after transfection with As-miR-21, the miR-21 expression in SHG-44(R) cells was knocked down significantly. As-miR-21 combined with radiation could synergistically enhanced mitotic death and apoptosis of SHG-44(R) cells, with SER of D(0) and D(q) being equal to 1.48 and 1.54, respectively. Expression levels of caspase-3 in the radiation group, AS-miR-21 transfection group and combination group were 2.24 ± 0.14, 2.05 ± 0.19 and 5.72 ± 0.45, respectively, and its expression in the combination group was higher than that in the other two groups.
CONCLUSIONSmiR-21 may be involved in the formation of radioresistance of glioma cells and as a potential target for enhancing the response of radioresistant-glioma cells to radiotheapy.
