Expression of hnRNPK in gastric carcinoma and its relationship with Helicobacter pylori L-form infection.
- Author:
Yan ZHAO
1
;
Xin JIN
;
Tian TIAN
;
Dong-hong YU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Female; Gastric Mucosa; metabolism; Helicobacter Infections; metabolism; microbiology; Helicobacter pylori; Heterogeneous-Nuclear Ribonucleoprotein K; Humans; Lymphatic Metastasis; Male; Middle Aged; Neoplasm Staging; Precancerous Conditions; metabolism; microbiology; pathology; RNA, Messenger; metabolism; Ribonucleoproteins; genetics; metabolism; Stomach Neoplasms; metabolism; microbiology; pathology
- From: Chinese Journal of Oncology 2011;33(10):759-763
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression feature of heterogeneous nuclear ribonucleoprotein K in gastric carcinoma and its clinical significance, and to explore the relationship between hnRNPK expression and Helicobacter pylori L-form infection.
METHODSThe expression of hnRNPK protein was examined in 100 cases of gastric carcinoma, 50 paracancerous gastric tissues and 30 matched normal gastric mucosa by Elivision immunohistochemistry and hnRNPK-mRNA by in situ hybridization. Hp-L was detected with Gram staining and immunohistochemical staining.
RESULTSThe positive rates of hnRNPK protein and mRNA in gastric carcinoma were 82.0% and 86.0%, respectively, significantly higher than those in the paracancerous gastric tissues and normal controls (P < 0.05). The expression of hnRNPK protein was significantly correlated with histological differentiation, TNM stage and lymph node metastasis (P < 0.05). The positive rates of Hp-L in the three groups were 67.0%, 58.0% and 23.3%, respectively. The positive rate of Hp-L in gastric carcinoma had no significant correlation with it in the paracancerous gastric tissues, but was significantly higher than it in the normal controls (P < 0.05). In gastric carcinoma, the expression of hnRNPK protein was higher in cases of Hp-L positive patients than those of Hp-L negative cases (P < 0.05). Positive correlation existed between the expression of hnRNPK protein and Hp-L infection (r = 0.391, P < 0.01).
CONCLUSIONSThere is a higher expression of hnRNPK in gastric carcinoma. Hp-L infection may be associated with the up-regulated hnRNPK expression. The two factors may play a synergetic role in gastric carcinogenesis.